Selected publications available by e-mail

Sreenath, Hassan K.; Jeffries, Thomas W.

1996. Biotechnol. Tech. 10(4): 239-242.

In this study, a variable-tilt microfuge fermentation rack to screen liquid cultures of wild and mutant yeasts for ethanol production was designed. The rack design allows for the evaluation of up to 40 cultures in the space normally required for three 125-mL flasks.

Fed-Batch Culture for Xylitol Production by Candida boidinii (PDF 195 Kbytes)

Vandeska, Eleonora; Amartey, S.; Kuzmanova, Slobodanka; Jeffries, T.W.

1996. Process Biochem. 31(3): 265-270.

In this study, Xylitol production by Candida boidinii NRRL Y-17213 was investigated in fed-batch fermentation with xylose (50, 100 g litre-1) and a mixture of glucose (25 g litre-1).

Amartey, Samuel; Jeffries, Thomas W.

1994. Biotechnol. Ltrs. 16(2): 211-214.

Results of this study show that a fermentation medium containing corn steep liquor as the sole source of nitrogen, vitamins, and other nutritional requirements compares favorably with several more complex media formulations for the fermentation of xylose to ethanol by P. stipitis CBS 6054.

Vandeska, E.; Amartey, S.; Kuzmanova, S.; Jeffries, T.

1995. World J. Microbiol. and Biotech. 11(2): 213-218.

The objective of this study was to screen a number of yeasts for even higher xylitol production; C. boidinii NRRL Y-17213 was identified as a promising xylitol producer and therefore selected for investigation of the effects of environmental conditions on its xylitol production.

Yang, Vina W.; Marks, Jere A.; Davis, Brian P.; Jeffries, Thomas W.

1994. Appl. Environ. Microbiol. 60(12): 4245-4254.

This paper describes the first high-efficiency transformation system for the xylose-fermenting yeast Pichia stipitis. The system includes integrating and autonomously replicating plasmids based on the gene for orotidine-5 -phosphate decarboxylase (URA3) and an autonomous replicating sequence (ARS) element (ARS2) isolated from P.stipitis CBS 6054.